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By Maskur, Adi Sucipto

19 Jan 2010

Present status of common Carp Genetics and Breeding at Main Center for Freshwater Aquaculture Development, Sukabumi

I. Background

Indonesia is a country with a high level of biological diversity. There are approximately 1,050 freshwater fish species, of which about 950 species are found in western Indonesia and Sulawesi (Kottelat et al. 1993) and at least 103 species are found in Sahul Shelf (Schuster 1950). The fish species are characterized by their high levels of endemism. Kottelat et al. (1993) reported that in Sumatra there are 30 endemic species, 149 species in Kalimantan, 12 species in Java and 52 species in Sulawesi.

Promoter

Promoter berperan penting dalam pembuatan ikan transgenik. Promoter merupakan sekuen DNA yang terletak di bagian hulu (upstream, terminal 5) dari lokasi dimulainya transkripsi (Hackett 1993) yang berperan dalam mengatur letak, waktu dan tingkat ekspresi gen yang akan muncul (Beaumont & Hoare 2003). Daerah promoter adalah cis-acting; mereka mempengaruhi transkripsi dalam segmen DNA yang sama dimana mereka berada.